qPCR + Targeted 16S/ITS NGS Diagnostic Technology
DNA detection with the greatest clinical utility
MicroGenDX combines qPCR with targeted 16S/ITS NGS to deliver timely, highly accurate, actionable diagnostic information for infections. Matching samples against a curated database of over 50,000 microbial species, MicroGenDX’s qPCR+NGS results identify all potentially pathogenic microbial taxa, along with their clinically relevant distribution in the sample, in 3.5 days, at a low cost. In contrast, the strain-level resolution and discovery power of shotgun metagenomics does not provide any greater diagnostic utility for most infections* — and shotgun is also slower and more expensive, with lower sensitivity and accuracy than targeted NGS. For clinical applications, qPCR and targeted NGS offer a clear advantage for optimal treatment strategies.
Comparison of DNA sequencing technologies
|qPCR||Targeted 16S/ITS NGS||Shotgun Metagenomics**|
Sequence library is much smaller
*Shotgun metagenomics may discover additional resistance genes, but MicroGenDX qPCR detects a total of 17 resistance genes from among all main classes of antimicrobials.**MicroGenDX is also able to provide shotgun metagenomics offered by its RTL Genomics research division.
- Mitchell SL, Simner PJ. Next-Generation Sequencing in Clinical Microbiology: Are We There Yet? Clin Lab Med. 2019 Sep;39(3):405-418.doi: 10.1016/j.cll.2019.05.003.