Published Studies

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All Published Studies
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An antimicrobial prophylaxis protocol using rectal swab cultures for transrectal prostate biopsy

Aims

The diagnosis of periprosthetic joint infection can be difficult due to the high rate of culturenegative infections. The aim of this study was to assess the use of next-generation sequencing for detecting organisms in synovial fluid.

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Assessment of Prostate Cancer Risk in Men with PSA < 1.5

Aims

The diagnosis of periprosthetic joint infection can be difficult due to the high rate of culturenegative infections. The aim of this study was to assess the use of next-generation sequencing for detecting organisms in synovial fluid.

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"We have the option for 100% effective treatment." Beth Pearce, DPM
"We have the option for 100% effective treatment." An Interview with Beth Pearce, DPM
"We have been waiting for technology like this." Javad Parvizi, MD
"This made a significant difference in the patient's care and his outcome." Michael Waters, DO
"This is the best my sinus has been in my entire adult life." Laurence O'Halloran, MD talks about his patient's success using MicroGen DX
"You can identify it, you treat it, now you're able to better heal it." Matthew Regulski, DPM
The MicroGen DX Laboratory: Next-Gen Sequencing; Superior Diagnostics Simplified
"This is a huge change where we can diagnose these patients earlier." An interview with Michael McDonald, MD
Identifying the causative microbes of infections with Next-Gen Sequencing- Andrew Pugliese M.D.
"This revolutionized the way I practice the management of chronic infections" Andrew Pugliese, MD
Diagnosis of Streptococcus canis periprosthetic joint infection: the utility of next-generation sequencing

Aims

The diagnosis of periprosthetic joint infection can be difficult due to the high rate of culturenegative infections. The aim of this study was to assess the use of next-generation sequencing for detecting organisms in synovial fluid.

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A Pilot Study to Evaluate Next Generation DNA Sequencing Testing to Rectal Swabs

Aims

The diagnosis of periprosthetic joint infection can be difficult due to the high rate of culturenegative infections. The aim of this study was to assess the use of next-generation sequencing for detecting organisms in synovial fluid.

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Diagnosis of Periprosthetic Joint Infection: The Potential of Next-Generation Sequencing

Aims

The diagnosis of periprosthetic joint infection can be difficult due to the high rate of culturenegative infections. The aim of this study was to assess the use of next-generation sequencing for detecting organisms in synovial fluid.

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More than meets the eye: Innovative technology spots bacteria previously undetected
Can next generation sequencing play a role in detecting pathogens in synovial fluid?

Aims

The diagnosis of periprosthetic joint infection can be difficult due to the high rate of culturenegative infections. The aim of this study was to assess the use of next-generation sequencing for detecting organisms in synovial fluid.

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"We started doing the DNA sequencing... and were AMAZED." Michael Lavor, MD
"I trust it implicitly" Margarita Johnson, MD
"MicroGen DX gives us the right key." Nabeel Syed, MD
“With this test, you’re going to be the hero.” Roscoe Nelson, MD
"With this test, you're going to be the hero." Roscoe Nelson, MD
“I feel proud that I can get this treatment to the patients.” Lisa Zambrano, NP
"I feel proud that I can get this treatment to the patients." Lisa Zambrano, NP
“It’s inordinately valuable for patients.” Keith Matheny, MD
"It's inordinately valuable for patients." Keith Matheny, MD
“It’s a win for me, it’s a win for the patient.” Lyle Haskell, D.P.M
"It's a win for me, it's a win for the patient." Lyle Haskell, D.P.M
“Truly, it’s precision medicine.” Robert Dean, MD
"Truly, it's precision medicine." Robert Dean, MD
“MicroGen DX is absolutely a key part of our whole management strategy.” Jon Minter, DO
"MicroGen DX is absolutely a key part of our whole management strategy." Jon Minter, DO
“It is going help me, help my patients.” Ginger Isom-Batz, MD
"It is going help me, help my patients." Ginger Isom-Batz, MD
“This has revolutionized my practice, it will revolutionize your practice.” Timothy Hlavinka, MD
"This has revolutionized my practice, it will revolutionize your practice." Timothy Hlavinka, MD
AUA News

Aims

The diagnosis of periprosthetic joint infection can be difficult due to the high rate of culturenegative infections. The aim of this study was to assess the use of next-generation sequencing for detecting organisms in synovial fluid.

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Comparative Phase II Study of Standard Urine C&S vs DNA NGS Testing for UTIs

Aims

The diagnosis of periprosthetic joint infection can be difficult due to the high rate of culturenegative infections. The aim of this study was to assess the use of next-generation sequencing for detecting organisms in synovial fluid.

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MicroGenDX featured on LifeTime Television
MicroGenDX featured on LifeTime Television
Live UTI Free on MicroGenDX
Use of Dipstick Assay and Rapid PCR-DNA Analysis of Nasal Secretions for Diagnosis of Bacterial Sinusitis in Children With Chronic Cough

Aims

The diagnosis of periprosthetic joint infection can be difficult due to the high rate of culturenegative infections. The aim of this study was to assess the use of next-generation sequencing for detecting organisms in synovial fluid.

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“Why aren’t you using this?” Eric L. Johnson, M.D.
General Assembly, Diagnosis, Pathogen Isolation: Proceedings of International Consensus on Orthopedic Infections

shown to be powerful tools in detecting prosthetic joint infections (PJIs) with negative cultures, although conflicting data exist on PCR. Sonication of explanted prosthetics can enhance both the sensitivity of conventional cultures and PCR.

Level of Evidence: Strong

Delegate Vote: Agree: 85%, Disagree: 9%, Abstain: 6% (Super Majority, Strong Consensus)

Pablo S. Corona, Karan Goswami, Naomi Kobayashi, William Li, Adolfo Llinas Volpe, Oliver Marín-Pe~na, Daniel Monsalvo, Fernando Motta, Alexander Shope, Majd Tarabichi, Matias Vicente, Hamidreza Yazdi

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Determining the utility of standard hospital microbiology testing: Comparing standard microbiology cultures with DNA sequence analysis in patients with chronic sinusitis

Results: Specimens were obtained from 29 female and 16 male patients with a mean age of 50 years. A total of 45 cultures were included in our final analysis; five cultures were excluded after inappropriate laboratory processing. Results from these patients were compared and analyzed. Cohen’s weighted kappa analysis showed agreement between the two testing

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Metagenomics in diagnosis and improved targeted treatment of UTI

Results: This paper is derived from an extensive review and analysis of the available literature on the topic of metagenomic sequencing in urological science, using the PubMed search engine. The search yielded a total of 406 results, and manual selection of appropriate papers was subsequently performed.

Matthew Dixon · Maria Stefil · Michael McDonald · Truls Erik Bjerklund‑Johansen · Kurt Naber · Florian Wagenlehner · Vladimir Mouraviev

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MicroGenDX's Next-Generation DNA Sequencing Ushers in New Era of Advanced Infection Diagnostics at 2019 American Academy of Orthopedic Surgeons with Multiple Podium Talks
NEXT GENERATION SEQUENCING OF MICROBIOMES IN POST-DRE URINE SAMPLES OF PROSTATE CANCER PATIENTS

Introduction : Pathogenic microorganisms could be responsible for asymptomatic and symptomatic inflammatory processes in the prostate including Escherichia coli, Pseudomonas spp., Neisseria gonorrhoeae, Trichomonas vaginalis, etc (1). Modifications of bacterial populations were observed according to different inflammatory and tumor conditions in prostate cancer (PCa) samples, which may promote the development of cancer by enhancing extracellular environmental factors within the prostate (2-4).

E. David Crawford, M.D, Rick Martin, Caleb D. Phillips, Ph.D., Whitney N. Stanton, Adrie van Bokhoven, Ph.D., M. Scott Lucia, M.D., Paul Arangua, Francisco G. La Rosa, M.D., Zachary Grasmick, Gretchen Hoyer, Vladimir Mouraviev, M.D., Ph.D., Nelson N. Stone, M.D., Priya N. Werahera, Ph.D.

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Evaluation of the bacterial diversity of pressure ulcers using bTEFAP pyrosequencing

BMC Medical Genomics

Drake M Smith1, David E Snow1, Eric Rees1, Ann M Zischkau1, J Delton Hanson1, Randall D Wolcott2, Yan Sun1, Jennifer White1, Shashi Kumar1, Scot E Dowd1,3*
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Evaluation of bacterial diversity in the feces of cattle using 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP)

BMC Microbiology

Scot E Dowd*1,3,4, Todd R Callaway2, Randall D Wolcott3, Yan Sun3, Trevor McKeehan3, Robert G Hagevoort5 and Thomas S Edrington2
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Evaluation of the bacterial diversity among and within individual venous leg ulcers using bacterial tag-encoded FLX and Titanium amplicon pyrosequencing and metagenomics approaches

BMC Microbiology

Randall D Wolcott1,2, Viktoria Gontcharova3, Yan Sun3 and Scot E Dowd*2,3
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Polymicrobial Nature of Chronic Diabetic Foot Ulcer Biofilm Infections Determined Using Bacterial Tag Encoded FLX Amplicon Pyrosequencing (bTEFAP)

PLoS One

Scot E. Dowd1,3*, Randall D. Wolcott2, Yan Sun1, Trevor McKeehan1, Ethan Smith3, Daniel Rhoads2
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Identification of yeast in chronic wounds using new pathogen-detection techniques

Journal of Wound Care, Vol.18, No.3

J.L. Leake, S.E. Dowd, R.D. Wolcott, A.M. Zischkau, Y. Sun
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In vitro multispecies Lubbock chronic wound biofilm model

Wound Repair and Regeneration

Yan Sun, PhD1; Scot E. Dowd, PhD1; Ethan Smith, BSc1; Dan D. Rhoads, MTC2; Randall D. Wolcott, MD2
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Survey of bacterial diversity in chronic wounds using Pyrosequencing, DGGE, and full ribosome shotgun sequencing

BMC Microbiology

Scot E Dowd*1, Yan Sun2, Patrick R Secor3, Daniel D Rhoads2, Benjamin M Wolcott2, Garth A James3 and Randall D Wolcott2
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Healing and healing rates of chronic wounds in the age of molecular pathogen diagnostics

Journal of Wound Care

Wolcott RD, Cox SB, Dowd SE
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Clinical identification of bacteria in human chronic wound infections: culturing vs 16S ribosomal DNA sequencing

BMC Infectious Diseases

Daniel D Rhoads1, Stephen B Cox2, Eric J Rees2, Yan Sun2 and Randall D Wolcott2,3,4*
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Molecular diagnostics and personalized medicine in wound care: assessment of outcomes

Journal of Wound Care, Vol.20, No.5

S.E. Dowd,1 PhD, Research Director; R.D. Wolcott,2 MD, Medical Director; J. Kennedy,3 PhD, R.Ph.; C. Jones,3 PhD, R.Ph; S.B. Cox,4 PhD
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Determining the utility of standard hospital microbiology testing: Comparing standard microbiology cultures with DNA sequence analysis in patients with chronic sinusitis

World Journal of Otorhinolaryngology Head and Neck Surgery

Sarah K. Rapoport a,*, Alyssa J. Smith b, Maxwell Bergman c,Kelly A.Scriven a, Itzhak Brook d, Suzette K. Mikula a
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Comparison of Culture and Molecular Identification of Bacteria in Chronic Wounds

International Journal of Molecular Sciences

Daniel D. Rhoads 1,2, Randall D. Wolcott 1,3,4,*, Yan Sun 3 and Scot E. Dowd 3,4
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Culture Enriched Molecular Profiling of the Cystic Fibrosis Airway Microbiome

PLoS One

Christopher D. Sibley1, Margot E. Grinwis1, Tyler R. Field1, Christina S. Eshaghurshan1, Monica M. Faria1, Scot E. Dowd4,5, Michael D. Parkins1,2,3, Harvey R. Rabin1,2,3, Michael G. Surette1,6*
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An in vivo Polymicrobial Biofilm Wound Infection Model to Study Interspecies Interactions

PLoS One

Trevor Dalton1,2, Scot E. Dowd3, Randall D. Wolcott3, Yan Sun3, Chase Watters1,2, John A. Griswold1, Kendra P. Rumbaugh1,2*
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Microbiota is a primary cause of pathogenesis of chronic wounds

Journal of Wound Care, Vol.25, No.10

Wolcott R1, Sanford N1, Gabrilska R2, Oates JL3, Wilkinson JE3, Rumbaugh KP2
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The presence of biofilm structures in atherosclerotic plaques of arteries from legs amputated as a complication of diabetic foot ulcers

Journal of Wound Care, Vol.25, No.2

D.E. Snow1, J. Everett2, G. Mayer1, S.B. Cox3, B. Miller5, K. Rumbaugh2, R.A. Wolcott3
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Analysis of the chronic wound microbiota of 2,963 patients by 16S rDNA pyrosequencing

Wound Repair and Regeneration

Randall D. Wolcott, MD1†; John D. Hanson, PhD2†; Eric J. Rees, PhD2†; Lawrence D. Koenig, PhD2†; Caleb D. Phillips, PhD2†; Richard A. Wolcott, PhD2,3†; Stephen B. Cox, PhD2†; Jennifer S. White, MS3†
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Comparative study of cultures and next-generation sequencing in the diagnosis of shoulder prosthetic joint infections

Journal of Shoulder and Elbow Surgery

Surena Namdari, MD, MSc*, Thema Nicholson, MSc, Joseph Abboud, MD, Mark Lazarus, MD, Matthew L. Ramsey, MD, Gerald Williams, MD, Javad Parvizi, MD
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Diagnosis of Periprosthetic Joint Infection: The Potential of Next-Generation Sequencing

The Journal of Bone and Joint Surgery, Inc.

Majd Tarabichi, MD, Noam Shohat, MD, Karan Goswami, MD, Abtin Alvand, MD, PhD, FRCS, Randi Silibovsky, MD, Katherine Belden, MD, and Javad Parvizi, MD, FRCS
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An implementation of next generation sequencing for prevention and diagnosis of urinary tract infection in urology

The Canadian Journal of Urology

Vladimir Mouraviev, MD1, Michael McDonald, MD2
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A Head-to-Head Comparative Phase II Study of Standard Urine Culture and Sensitivity Versus DNA Next-Generation Sequencing Testing for Urinary Tract Infections

Reviews in Urology, Vol.19, No.4

Michael McDonald, MD,1,2 Darian Kameh, MD,3 Mark E. Johnson, PhD,4 Truls E. Bjerklund Johansen, MD, DMSci,5 David Albala, MD,6 Vladimir Mouraviev, MD, PhD7,8
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MP15-14 The value of next generation DNA sequencing testing in rectal swabs before transrectal prostate biopsy for individual and targeted prophylaxis of urinary tract infection

The Journal of Urology, Vol.199, No.4S

Vladimir Mouraviev*, Davenport, FL; Michael W. McDonald, Celebration, FL; Colby Skinner, Orlando, FL; Srinivas Vourganti, Chicago, IL; David M. Albala, Syracuse, NY; Kurt Naber, Munich, Germany; Florian M. E. Wagenlehner, Giessen, Germany; Truls E. Bjerklund Johansen, Oslo, Norway
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MP23-06 An utilization of next generation sequencing of urine samples for monitoring of urinary tract infections in patients with neurogenic bladder

The Journal of Urology, Vol.199, No.4S

Yan Liu*, Ellen Shapiro, Herbert Lepor, Tung-Tien Sun, Xue-Ru Wu, New York, NY
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Metagenomics in diagnosis and improved targeted treatment of UTI

World Journal of Urology

Matthew Dixon1, Maria Stefil1, Michael McDonald2,3, Truls Erik Bjerklund‑Johansen4, Kurt Naber5, Florian Wagenlehner6, Vladimir Mouraviev2,3,7
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Can next generation sequencing play a role in detecting pathogens in synovial fluid?

The Bone & Joint Journal

Tarabichi, N. Shohat, K. Goswami, J. Parvizi
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Diagnosis of Streptococcus canis periprosthetic joint infection: the utility of next-generation sequencing

Arthroplasty Today

Majd Tarabichi, MD, Abtin Alvand, MD, PhD, FRCS (Tr&Orth), Noam Shohat, MD, Karan Goswami, MD, Javad Parvizi, MD, FRCS
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Use of Dipstick Assay and Rapid PCR-DNA Analysis of Nasal Secretions for Diagnosis of Bacterial Sinusitis in Children With Chronic Cough

Allergy and Rhinology

Charles Song, MD1, Jeena Chorath, MD1, Youngju Pak, PhD1, and Nasser Redjal, MD1
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The Potential of High-Throughput DNA Sequencing of the Paranasal Sinus Microbiome in Diagnosing Odontogenic Sinusitis

American Academy of Otolaryngology–Head and Neck Surgery Foundation

Asad A. Haider1, Michael J. Marino, MD2, William C. Yao, MD1, Martin J. Citardi, MD1, and Amber U. Luong, MD, PhD1,3
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MP47-07 Comparative Value of Chronic Urinary Tract Infection (UTI) Diagnosis Between Standard Culture Sensitivity and Next Generation Sequence (NGS) in Urine Samples

The Journal of Urology, Vol.201, No.4S

George Coba*, Timothy Koo, Saif Zaman, Tampa, FL; Maria Stefil, Matthew Dixon, Norwich, United Kingdom; Liqiang Ni, Orlando, FL; Michael McDonald, Celebration, FL; Vladimir Mouraviev, Orlando, FL
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MP71-03 Comparison of Novel DNA-Based Tools for Bacterial Detection in Patients with Chronic Urinary Tract Infection (CUTI)

The Journal of Urology, Vol.201, No.4S

George Coba*, Timothy Koo, Saif Zaman, Tampa, FL; Maria Stefil, Matthew Dixon, Norwich, United Kingdom; Liqiang Ni, Orlando, FL; Michael McDonald, Celebration, FL; Vladimir Mouraviev, Orlando, FL
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