ENT | Understanding Your Report Results

Interpreting Your qPCR+NGS Level 2 Report

The normal sinus microbiome is rich in diversity with a large number (6-10) of microbes
A diseased state exhibits less biodiversity, less richness, and less evenness
The normal sinus microbiome tends to be dominated by Staph epi and/or C. acnes
Pathogens can be present in a healthy microbiome, but in low abundances
Select bacteria – such as Staph aures and anaerobes like Prevotella, Fusobacterium, Bacteroides spp. and Peptostreptococcus spp. – are more abundant in CRS diseased sinuses

Some percentage of healthy middle ear have a bacterial microbiome, though with a low bioburden
A healthy middle ear microbiome can contain pathogens such as Staphylococcus, Pseudomonas, Streptococcus, and Moraxella
A healthy middle ear microbiome has a higher abundance of Cutibacterium

Aminoglycoside resistance genes are present in the sample.
Total bacterial load is medium (10⁵ - 10⁷ per mL)
A complete list of clinically-relevant microbes detected in the sample:
1. qPCR detected Streptococcus pneumoniae at a low load (7.19 x 10⁴);
2. NGS detected Prevotella melaninogenica as the dominant species at 78%, along with two other anaerobes, making up 90% of the total bacterial load;
3. The microbial abundance distribution informs treatment decisions.
Antimicrobials for consideration, based on Sanford Guide and Johns Hopkins Guides (Infectious Disease consultations also available). Not determined by phenotypic susceptibility analysis.